Immunological identification and sequence characterization of a peptide derived from the processing of neuroendocrine protein 7B2

FEBS Letters

Chrétien, Michel¹  Mbikay, Majambu¹  Rondeau, Normand¹  Lazure, Claude¹  Seidah, Nabil G.¹  Paquet, Luc¹ 

[1] Institut de Recherches Cliniques de Montréal, Université de Montréal, 110 avenue des Pins Ouest, Montréal, Québec, H2W 1R7, Canada

关键词: 7B2;    Precursor protein;    Antibody;    Proteolytic processing;    AtT-20 cell;   

DOI  :  10.1016/0014-5793(91)81334-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A newly raised antiserum against the C-terminal region of neuroendocrine protein 7B2 was used to purify a novel peptide from the culture media of the mouse corticotroph cell line AtT-20. Based on partial sequencing, this peptide, which we call Cter-7B2, begins at Ser¹⁵⁶ and appears to result from the cleavage of pro7B2 after a five-basic-residue sequence. Thus, 7B2 processing may contribute to the diversity of peptides found in neuronal and endocrine cells.

【 授权许可】

Unknown