Interleukin 1 receptor antagonist in human epidermis and cultured keratinocytes

FEBS Letters

Dayer, J.-M.¹  Baumberger, C.¹  Gruaz-Chatellard, D.²  Saurat, J.-H.² 

[1] Division of Immunology and Allergy, Department of Medicine, Hôpital Cantonal Universitaire, 1211 Genève 4, Switzerland;Clinic of Dermatology, Department of Medicine, Hôpital Cantonal Universitaire, 1211 Genève 4, Switzerland

关键词: Keratinocyte;    Human epidermis;    Interleukin-1;    Interleukin-1 receptor antagonist;   

DOI  :  10.1016/0014-5793(91)81360-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin 1 (IL-1), present in high amounts in normal human skin without any sign of inflammation, suggests a complex mechanism by which its bioactivity is regulated. The specific receptor antagonist of IL-1 (IL-1ra) was analyzed in human skin, sweat and cultured keratinocytes. Extracts of both skin and cultured keratinocytes blocked the binding of [¹²⁵I]IL-1 to its receptor whereas sweat did not. The inhibitory activity was cell-associated, was not secreted by cultured keratinocytes, and IL-1ra mRNA was identified in these cells. There was an inverse relationship between the level of IL-1ra and that of IL-1α and β since extracts of differentiating keratinocytes (DK) and higher IL-1ra levels and expressed more mRNA for IL-1ra than non-differentiated keratinocytes (NDK), whereas NDK contained 4 times more IL-1α and β proteins than DK. This association of cell differentiation with a shift in agonist/antagonist ratio might be related to important autocrine or paracrine functions of IL-1 in normal and inflamed human skin.

【 授权许可】

Unknown