FEBS Letters | |
Coexpression of type I and type II IL‐1 receptors in the murine T helper 2 cell line D10N | |
Resch, K.2  Brigelius-Flohé, R.2  Szamel, M.2  Martin, M.2  McCarthy, J.E.G.1  | |
[1] Gesellschaft für Biotechnologische Forschung (GBF), Braunschweig, Germany;Medizinische Hochschule Hannover, Institut für Molekularpharmakologie, Hannover, Germany | |
关键词: Interleukin-1 receptor; Interleukin-1; Interleukin-1 receptor antagonist; Mouse T helper cell; | |
DOI : 10.1016/0014-5793(93)81791-W | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
IL-1 receptor heterogeneity in murine lymphocytes was investigated by cross-linking to [125I]IL-1α and competition with IL-1 receptor antagonist, and the molecular identity of the IL-1 receptors was identified with PCR using primers specific for type I and type II IL-1 receptors. The thymoma cell line EI4 6.1 exhibits exclusively the 80 kDa receptors which proved to be the type I receptor according to PCR analysis. In the pre-B cell line 70Z/3, predominantly a 60 kDa type II receptor but also a trace of type I receptor can be identified by PCR. The Th2 cell line D10N expresses both types of IL-1 receptors in equivalent amounts according to cross-linking experiments and PCR. The proliferative response of D10N cells to IL-1 is inhibited by IL-1 ra which according to cross-linking affects the binding to the type I receptor only. It is concluded that coexpression of both types of IL-1 receptors might be a characteristic of murine Th2 cells and that their growth-dependence on IL-1 is mediated by the type I receptor.
【 授权许可】
Unknown
【 预 览 】
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