Coexpression of type I and type II IL‐1 receptors in the murine T helper 2 cell line D10N

FEBS Letters

Resch, K.²  Brigelius-Flohé, R.²  Szamel, M.²  Martin, M.²  McCarthy, J.E.G.¹ 

[1] Gesellschaft für Biotechnologische Forschung (GBF), Braunschweig, Germany;Medizinische Hochschule Hannover, Institut für Molekularpharmakologie, Hannover, Germany

关键词: Interleukin-1 receptor;    Interleukin-1;    Interleukin-1 receptor antagonist;    Mouse T helper cell;   

DOI  :  10.1016/0014-5793(93)81791-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

IL-1 receptor heterogeneity in murine lymphocytes was investigated by cross-linking to [¹²⁵I]IL-1α and competition with IL-1 receptor antagonist, and the molecular identity of the IL-1 receptors was identified with PCR using primers specific for type I and type II IL-1 receptors. The thymoma cell line EI4 6.1 exhibits exclusively the 80 kDa receptors which proved to be the type I receptor according to PCR analysis. In the pre-B cell line 70Z/3, predominantly a 60 kDa type II receptor but also a trace of type I receptor can be identified by PCR. The Th2 cell line D10N expresses both types of IL-1 receptors in equivalent amounts according to cross-linking experiments and PCR. The proliferative response of D10N cells to IL-1 is inhibited by IL-1 ra which according to cross-linking affects the binding to the type I receptor only. It is concluded that coexpression of both types of IL-1 receptors might be a characteristic of murine Th2 cells and that their growth-dependence on IL-1 is mediated by the type I receptor.

【 授权许可】

Unknown