Crystals of soluble interleukin‐1 receptor complexed with its natural antagonist reveal a 1:1 receptor‐ligand complex

FEBS Letters

Barrett, Ronald W.¹  Bowlin, Terry²  Yanofsky, Stephen¹  Sarubbi, Edoardo⁴  Akeson, Ann²  Schreuder, Herman³  Soffientini, Adolfo⁴  Tardif, Chantal³ 

[1] Affymax Research Institute, Palo Alto, CA 94304, USA;Marion Merrell Dow Research Institute, Cincinnati, OH 45242-9553, USA;Marion Merrell Dow Research Institute, 16 Rue d'Ankara, 67080 Strasbourg Cedex, France;Lepetit Research Center, 21040 Gerenzano Varese, Italy

关键词: Interleukin-1 receptor;    Interleukin-1 receptor antagonist;    Cytokine;    Protein crystal;    X-Ray analysis;    Human;    IL-1;    interleukin-1;    IL-1ra;    interleukin-1 receptor antagonist;   

DOI  :  10.1016/0014-5793(95)01009-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interleukin-1 is a cytokine involved in the acute phase response against infection and injury. We obtained crystals of a complex of soluble, recombinant human interleukin-1 receptor and recombinant human interleukin-1 receptor antagonist, a naturally occurring antagonist. The crystals are suitable for X-ray analysis and diffract to 2.7 Å resolution. Solvent content calculations indicate that the crystals contain one receptor and one antagonist molecule per asymmetric unit. Other receptor to antagonist ratios are highly unlikely. These results suggest that the interleukin-1 antagonist binds a single receptor molecule and does not cause receptor aggregation.

【 授权许可】

Unknown