Dual regulation of the T‐type Ca2+ current by serum albumin and β‐estradiol in mammalian spermatogenic cells

FEBS Letters

Darszon, Alberto¹  Felix, Ricardo¹  Kopf, Gregory S.²  Espinosa, Felipe¹  Visconti, Pablo E.³  De la Vega-Beltrán, Jose L.¹  Muñoz-Garay, Carlos¹  López-González, Ignacio¹ 

[1] Department of Genetics and Molecular Physiology, Institute of Biotechnology, UNAM, Avenida Universidad 2001, Col. Chamilpa, P.O. Box 62100, Cuernavaca, Mor., Mexico;Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, Philadelphia, PA, USA;Department of Anatomy and Cell Biology, University of Virginia, Charlottesville, VA, USA

关键词: T-Ca2+ channel;    Spermatogenic cell;    Serum albumin;    Bovine serum albumin;    β-Estradiol;    Sperm capacitation;   

DOI  :  10.1016/S0014-5793(00)01688-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study provides evidence for a novel mechanism of voltage-gated Ca²⁺ channel regulation in mammalian spermatogenic cells by two agents that affect sperm capacitation and the acrosome reaction (AR). Patch-clamp experiments demonstrated that serum albumin induced an increase in Ca²⁺ T current density in a concentration-dependent manner, and significant shifts in the voltage dependence of both steady-state activation and inactivation of the channels. These actions were not related to the ability of albumin to remove cholesterol from the membrane. In contrast, β-estradiol significantly inhibited Ca²⁺ channel activity in a concentration-dependent and essentially voltage-independent fashion. In mature sperm this dual regulation may influence capacitation and/or the AR.

【 授权许可】

Unknown