Study of Interaction between Cadmium and Bovine Serum Albumin with UV-Vis Spectrocopy Approach

International Conference on Nanomaterials and Biomaterials 2017

材料科学;物理学;生物科学

Suhartono, E.^1 ; Thalib, I.^2 ; Aflanie, I.^3 ; Noor, Z.^4 ; Idroes, R.^5

Department of Medical Chemistry/Biochemistry, Faculty of Medicine, Lambung Mangkurat University, South Kalimantan, Banjarmasin

70712, Indonesia^1

Research and Development Unit, Mutiara Bunda Mother and Child Hospital, Martapura, South Kalimantan

70614, Indonesia^2

Department of Forensic, Faculty of Medicine, Lambung Mangkurat University, South Kalimantan, Banjarmasin

70232, Indonesia^3

Department of Orthopaedic Surgery, Faculty of Medicine, Lambung Mangkurat University, South Kalimantan, Banjarmasin, Indonesia^4

Department of Chemistry/Pharmacy, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Aceh

24415, Indonesia^5

关键词: Absorbance data;    Bovine serum albumins;    Cd concentrations;    Constant values;    Graphical forms;    Metal binding;    Protein structures;    Serum albumin;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/350/1/012008/pdf
DOI  :  10.1088/1757-899X/350/1/012008

来源: IOP

【 摘 要 】

This study aims to explain the interaction of cadmium (Cd) with serum albumin through visible light (UV-Vis) spectroscopy approach. This study is an in vitro experimental study using Cd with several concentrations and Bovine Serum Albumin (BSA). Each solution was then incubated for 10 min at 37°C, and measured the absorbance at 220-300 nm. The absorbance data is then presented in graphical form. From the graph, a linear equation will appear to calculate the value of metal binding constants (K) to proteins. Also, in this present study we analsyed the ratio between A220 and A220 to identify changes in the protein region especially tyrosine and peptide bonds. The results show that the addition of Cd in different concentrations could increase the absorbance with a constant value (K) = 1.634. Based on the result, it seems the addition of Cd in different concentrations will lead the reaction to form BSA-Cd. Also, the result shows that the ration of A220/A280 were decreased with the increasing of Cd concentration. In conclusion, the addition of Cd could interact and changes the protein structure in BSA.