Identification of an optimal Ncx binding sequence required for transcriptional activation

FEBS Letters

Shimizu, Hironori¹  Hatano, Masahiko¹  Tokuhisa, Takeshi¹  Ichinose, Masaharu²  Kang, Myengmo¹  Iitsuka, Yoshinori¹ 

[1] Department of Developmental Genetics, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan;Department of Plastic and Reconstructive Surgery, Chiba University School of Medicine, Chiba 260-8670, Japan

关键词: Ncx;    Hox11;    DNA-binding site;    Homeobox protein;   

DOI  :  10.1016/S0014-5793(00)01651-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Ncx gene encodes a homeobox containing transcription factor that belongs to the Hox11 gene family. We determined specific Ncx protein binding consensus DNA sequences. Optimal Ncx binding sequences were 5′-CGGTAATTGG-3′ (TAAT core) and 5′-CGGTAAGTGG-3′ (TAAG core), which coincided with the Hox11 binding sequence. Both Ncx and Hox11 could bind to the TAAT and the TAAG core oligonucleotide in vitro. However, they could efficiently transactivate the reporter plasmid linked to the TAAT core sequence but not to the TAAG core sequence. Thus, Ncx and Hox11 act as transcriptional activators via their target sequence, 5′-CGGTAATTGG-3′.

【 授权许可】

Unknown