Expression of calcium‐buffering proteins in rat intrinsic laryngeal muscles

Physiological Reports

Renato Ferretti¹  Maria Julia Marques²  Tejvir S. Khurana³ 

[1] Departamento de Anatomia, Instituto de Biociencias de Botucatu, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil;Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil;Department of Physiology, Perelman School of Medicine and Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, Pennsylvania

关键词: Calcium;    laryngeal muscles;    Ncx;    Pmca1;    Serca;    store‐operated calcium entry;   

DOI  :  10.14814/phy2.12409

来源: Wiley

【 摘 要 】

Abstract

Intrinsic laryngeal muscles (ILM) are highly specialized muscles involved in phonation and airway protection, with unique properties that allow them to perform extremely rapid contractions and to escape from damage in muscle dystrophy. Due to that, they may differ from limb muscles in several physiological aspects. Because a better ability to handle intracellular calcium has been suggested to explain ILM unique properties, we hypothesized that the profile of the proteins that regulate calcium levels in ILM is different from that in a limb muscle. Calcium-related proteins were analyzed in the ILM, cricothyroid (CT), and tibialis anterior (TA) muscles from male Sprague–Dawley rats (8 weeks of age) using quantitative PCR and western blotting. Higher expression of key Ca²⁺ regulatory proteins was detected in ILM compared to TA, such as the sarcoplasmic reticulum (SR) Ca²⁺-reuptake proteins (Sercas 1 and 2), the Na⁺/Ca²⁺ exchanger, phospholamban, and the Ca²⁺-binding protein calsequestrin. Parvalbumin, calmodulin and the ATPase, Ca²⁺-transporting, and plasma membrane 1 were also expressed at higher levels in ILM compared to TA. The store-operated calcium entry channel molecule was decreased in ILM compared to the limb muscle and the voltage-dependent L-type and ryanodine receptor were expressed at similar levels in ILM and TA. These results show that ILM have a calcium regulation system profile suggestive of a better ability to handle calcium changes in comparison to limb muscles, and this may provide a mechanistic insight for their unique pathophysiological properties.

【 授权许可】

CC BY   
© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

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