Identification and expression of six family genes in mouse retina

FEBS Letters

Ohto, Hiromi²  Takizawa, Toshihiro¹  Kawakami, Kiyoshi²  Saito, Takuma¹ 

[1] Department of Anatomy, Jichi Medical School, Minamikawachi-machi, Kawachi-gun, Tochigi-ken, 329-04, Japan;Department of Biology, Jichi Medical School, Minamikawachi-machi, Kawachi-gun, Tochigi-ken, 329-04, Japan

关键词: Six gene family;    cDNA cloning;    Retina development;    Homeobox protein;    Gel retardation assay;    GST-fusion protein;    SIX;    sine oculis related homeobox;    Na+-ATPase;    sodium-dependent adenosine triphosphatase;    K+-ATPase;    potassium-dependent adenosine triphosphatase;    PBS;    phosphate-buffered saline;    GST;    glutathione S-transferase;    ARE;    Atplal regulatory element;   

DOI  :  10.1016/0014-5793(96)00899-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We identified five cDNA clones of the Six gene family which are expressed in retina. They are Six2, Six3α and Six3β (which are derived from alternative splicing forms), Six5, and AREC3/Six4. All of these Six family genes possess extensive sequence similarity among each other in the so-homologous region (Six domain and homeodomain) but differ greatly in structure in some other regions. The amino acid sequence similarity of the so-homologous region to the previously identified AREC3/Six4 is 70.1% for Six2, 57.3% for Six3α and Six3β, and 70.3% for Six5. The expression of these genes was observed in inner and outer nuclear layer, ganglion cell layer, and pigment epithelium of mouse retina by in situ hybridization. The so-homologous region of each Six family protein has specific DNA binding activity. Six5 and Six2 bind to the same sequence as does AREC3/Six4, while Six3 does not. These observations suggest that some of the Six family genes can regulate the same target genes.

【 授权许可】

Unknown