Involvement of poly(ADP‐ribose) polymerase and activation of caspase‐3‐like protease in heat shock‐induced apoptosis in tobacco suspension cells

FEBS Letters

Zhang, Gui-You¹  Yan, Chang-Hui¹  Dai, Yao-Ren¹  Tian, Rui-Hua¹ 

[1] Department of Biology, Tsinghua University, Beijing 100084, China

关键词: Poly(ADP-ribose) polymerase;    Apoptosis;    Caspase-3-like protease;    3-Aminobenzamide;    Nicotinamide;    Tobacco;    PARP;    poly(ADP-ribose) polymerase;    HS;    heat shock;    3-AB;    3-aminobenzamide;    NIC;    nicotinamide;    TUNEL;    terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling;    casp;    caspase;   

DOI  :  10.1016/S0014-5793(00)01561-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cleavage of poly(ADP-ribose) polymerase (PARP) by caspase (casp)-3 is an essential link in the apoptotic pathway in animal cells. In plant cells, however, there is no authentic evidence for the similar role that PARP may play during apoptosis. Using a heat shock (HS)-induced apoptosis system of tobacco cells, we found that immediately after a 4 h heat treatment, PARP was cleaved to form an 89 kDa signature fragment, while DNA laddering appeared only after a 20 h recovery following the HS. An activation of casp-3-like protease was also observed. The results suggest that apoptosis in plants and animals may share common mechanisms. On the other hand, when cells were preincubated with 4 mM 3-aminobenzamide or 2–8 mM nicotinamide, the specific inhibitors of PARP, before HS treatment, apoptotic cell death was reduced significantly. Our results thus imply that PARP may also be involved in apoptosis in a different way from the casp-related events.

【 授权许可】

Unknown