Ribonucleotide reductase is regulated via the R2 subunit during the life cycle of Trypanosoma brucei

FEBS Letters

Krauth-Siegel, R.Luise¹  Steverding, Dietmar²  Breidbach, Tanja² 

[1] Biochemie-Zentrum, Ruprecht-Karls-Universität, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany;Abteilung Parasitologie, Hygiene-Institut der Ruprecht-Karls-Universität, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany

关键词: Ribonucleotide reductase;    Differentiation;    Life cycle;    Cell cycle;    Trypanosoma brucei;    CB1-gp;    major lysosomal membrane protein;    DIG;    digoxigenin;    PARP;    procyclic acidic repetitive protein;    R1;    large subunit of ribonucleotide reductase;    R2;    small subunit of ribonucleotide reductase;    VSG;    variant surface glycoprotein;   

DOI  :  10.1016/S0014-5793(00)01533-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the occurrence of the R1 and R2 subunits of ribonucleotide reductase during the life cycle of Trypanosoma brucei. Whereas the R1 protein is present throughout the life cycle, the R2 protein is not found in cell cycle-arrested short stumpy trypanosomes. RT-PCR/hybridization analysis revealed almost equal amounts of the R1 and R2 mRNAs in all life cycle stages of the parasite. The data indicate that ribonucleotide reductase of African trypanosomes is developmentally controlled by post-transcriptional regulation of the R2 subunit.

【 授权许可】

Unknown