The inhibitory effect of the 5′ untranslated region of muscle acylphosphatase mRNA on protein expression is relieved during cell differentiation

FEBS Letters

Raugei, Giovanni¹  Ramponi, Giampietro¹  Fiaschi, Tania¹  Veggi, Daniele¹  Chiarugi, Paola¹ 

[1] Dipartimento di Scienze Biochimiche, Università di Firenze, viale Morgagni 50, 50134 Florence, Italy

关键词: Acylphosphatase;    5′ Untranslated region;    Enhanced green fluorescent protein;    Aphidicolin;    Phorbol 12-myristate 13-acetate;    Differentiation;    MT;    muscular type isoform of acylphosphatase;    5′UTR;    5′ untranslated region;    EGFP;    enhanced green fluorescent protein;    TPA;    phorbol 12-myristate 13-acetate;   

DOI  :  10.1016/S0014-5793(00)01496-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous experiments suggested that the upstream AUG triplet present in the 5′ untranslated region (UTR) of muscle acylphosphatase mRNA is involved in the regulation of protein expression. In this paper, we study the involvement of the 5′UTR secondary structure and upstream peptide on mRNA stability and protein translation. Our data, obtained using deletion and frame-shift mutants, demonstrate that the 5′UTR controls protein expression regulating translation together with mRNA stability. Furthermore, we demonstrate that the inhibitory effect of the 5′UTR of muscle acylphosphatase is relieved during the differentiation process in agreement with previous data reporting an increase of acylphosphatase content during cell differentiation. Finally, UV cross-linking experiments show that specific mRNA-binding proteins are associated with the 5′UTR of the muscle acylphosphatase mRNA.

【 授权许可】

Unknown