A single nucleotide is a sufficient 5′ untranslated region for translation in an eukaryotic in vitro system

FEBS Letters

Andrews, David W¹  Hughes, Martin J.G¹ 

[1] Dept. of Biochemistry, McMaster University, 1200 Main Street West, Hamilton, ON L8N 3Z5, Canada

关键词: Translation initiation;    5′ Untranslated region;    Consensus sequence;    Bax;    Bcl-2 associated protein x;    BPRL;    2 copies of the first 15 amino acids of Bax fused to the N-terminal of PRL;    BPRL0 frame;    product formed by translation of the BPRL gene from the authentic AUG codon;    BPRL+1 frame;    product formed by translation of the BPRL gene from an AUG situated 1 nucleotide from the 5′ cap of the RNA;    PRL;    bovine pre-prolactin;    PRL0 frame;    product formed by translation of the PRL gene from the authentic AUG codon;    PRL+1 frame;    product formed by translation of the PRL gene from an AUG situated 1 nucleotide from the 5′ cap of the RNA;    UTK;    high-expression 5′-UTR based on that of Xenopus β-globin;   

DOI  :  10.1016/S0014-5793(97)00965-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 5′ untranslated region of an RNA molecule is thought to play an important role in the regulation of translation. Following a recent report that a single nucleotide is sufficient to act in this role in the unicellular organism Giardia, we show that this is also the case for a mammalian in vitro system. These results also demonstrate that an RNA can initiate translation from a start codon where an ideal translational consensus sequence is impossible.

【 授权许可】

Unknown