The distribution of T‐DNA in the genomes of transgenic Arabidopsis and rice

FEBS Letters

Delseny, Michel¹  Guiderdoni, Emmanuel³  Mestre-Ortega, Dominique¹  Sallaud, Christophe³  Gallois, Patrick¹  Raynal, Monique¹  Barakat, Abdelali¹  Bernardi, Giorgio² 

[1] Laboratoire de Biologie Moléculaire et Physiologie Végétale, Université de Perpignan, 52 Avenue Villeneuve, 66000 Perpignan, France;Stazione Zoologica Anton Dohrn, Laboratorio di Evoluzione Molecolare, Villa Comunale, 80121 Naples, Italy;Centre de Coopération Internationale en Recherche Agronomique pour le Developpement, Montpellier, France

关键词: Isochore;    Plant;    Gene;    BAC;    bacterial artificial chromosome;    BAMD;    3;    6-bis(acetatomercurimethyl)-1;    4-dioxane;    GC;    molar fraction of guanine+cytosine in DNA;    GUS;    iudA;    T-DNA;    transferred DNA;   

DOI  :  10.1016/S0014-5793(00)01393-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Almost all the nuclear genes of four Gramineae (maize, wheat, barley, rice) and pea are located in DNA fractions covering only a 1–2% GC range and representing between 10 and 25% of the different genomes. These DNA fractions comprise large gene-rich regions (collectively called the ‘gene space’) separated by vast gene-empty, repeated sequences. In contrast, in Arabidopsis thaliana, genes are distributed in DNA fractions covering an 8% GC range and representing 85% of the genome. Here, we investigated the integration of a transferred DNA (T-DNA) in the genomes of Arabidopsis and rice and found different patterns of integration, which are correlated with the different gene distributions. While T-DNA integrates essentially everywhere in the Arabidopsis genome, integration was detected only in the gene space, namely in the gene-rich, transcriptionally active, regions of the rice genome. The implications of these results for the integration of foreign DNA are discussed.

【 授权许可】

Unknown