Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity

FEBS Letters

Wang, Min Xia³  Wang, Jian¹  Wang, Xing Li¹  Trudinger, Brian²  Murrell, George A.C.³  Sim, Ah Siew⁴ 

[1] Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 760549, San Antonio, TX 78245-0549, USA;Department of Obstetrics and Gynaecology, Westmead Hospital, University of Sydney, Sydney, N.S.W., Australia;Orthopaedic Research Institute, St. George Hospital, University of New South Wales, Sydney, N.S.W., Australia;Cardiovascular Genetics Laboratory, Prince of Wales Hospital, Sydney, N.S.W., Australia

关键词: Atherosclerosis;    Endothelial nitric oxide synthase polymorphism;    Endothelial nitric oxide synthase enzyme activity;    Cigarette smoking;    Gene–environment interaction;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    iNOS;    inducible nitric oxide synthase;    eNOS;    endothelial nitric oxide synthase;    nNOS;    neuronal nitric oxide synthase;    CAD;    coronary artery disease;   

DOI  :  10.1016/S0014-5793(00)01356-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We explored the interactive effects of endothelial nitric oxide synthase (eNOS) genotypes and cigarette smoking on protein levels and enzyme activity in 33 postpartum placentas. Whilst the eNOS protein levels were lower in the rare allele (0.48±0.11, n=9 vs. 1.05±0.10, n=24, P<0.01), the eNOS enzyme activity was about 7-fold higher in the rare allele (4556.2±255.4 vs. 621.8±180.5 cpm/mg/min, P<0.01). Smokers had lower eNOS protein levels (1.07±0.09 vs. 0.50±0.19, P<0.05) in both alleles. It reduced the eNOS activities only in the rare allele (non-smokers: 6143.8±251.2, n=5, smokers: 2968.5±259.4, n=4, 52% reduction, P<0.01). We conclude that associations between eNOS polymorphism and protein levels and enzyme activities are modifiable by smoking, the effects of smoking are dependent on the eNOS genotypes.

【 授权许可】

Unknown