Chronic ethanol ingestion increases efficiency of oxidative phosphorylation in rat liver mitochondria

FEBS Letters

Rigoulet, M²  Roulet, M¹  Fontaine, E³  Leverve, X.M³  Devin, A²  Sibille, B³  Piquet, M.A³  Filippi, C³  Nogueira, V³ 

[1] Laboratoire de Nutrition Clinique, CHUV, 1011 Lausanne, Switzerland;Institut de Biochimie et de Génétique Cellulaires, CNRS, 1 rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France;Laboratoire de Bioénergétique Fondamentale et Appliquée, UJF, P.O. Box 53X, 38041 Grenoble, France

关键词: Mitochondrion;    Oxidative phosphorylation;    Ethanol;    Cytochrome oxidase;    Proton-motive force;    Sodium azide;   

DOI  :  10.1016/S0014-5793(00)01225-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The efficiency of oxidative phosphorylation was compared between rats chronically fed with ethanol and controls. (i) Results showed that the liver mitochondria state 4 respiratory rate was strongly inhibited, while the corresponding proton-motive force was not affected; (ii) the cytochrome oxidase content and activity were decreased and (iii) the oxidative-phosphorylation yield was increased in the ethanol exposed group. Furthermore, oxidative phosphorylation at coupling site II was not affected by ethanol. Cytochrome oxidase inhibition by sodium-azide mimicked the effects of ethanol intoxication in control mitochondria. This indicates that the decrease in cytochrome oxidase activity induced by ethanol intoxication directly increases the efficiency of oxidative phosphorylation.

【 授权许可】

Unknown