Identification of lysophospholipid receptors in human platelets: the relation of two agonists, lysophosphatidic acid and sphingosine 1‐phosphate

FEBS Letters

Yatomi, Yutaka¹  Igarashi, Yasuyuki²  Motohashi, Ken²  Shibata, Satomi²  Ozaki, Yukio¹ 

[1] Department of Laboratory Medicine, Yamanashi Medical University, Nakakoma, Yamanashi 409-3898, Japan;Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12-Nishi-6, Kita-ku, Sapporo 060-0812, Japan

关键词: Edg family;    G-protein-coupled receptor;    Lysophosphatidic acid;    Platelet;    PSP24;    Sphingosine 1-phosphate;    LPA;    lysophosphatidic acid;    PCR;    polymerase chain reaction;    PMN;    polymorphonuclear;    RT;    reverse transcriptase;    Sph-1-P;    sphingosine 1-phosphate;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/S0014-5793(00)01222-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (Sph-1-P) are known as structurally related bio-active lipids activating platelets through their respective receptors. Although the receptors for LPA and Sph-1-P have been recently identified in various cells, the identification and characterization of ones in platelets have been reported only preliminarily. In this report, we first investigated the distinct modes of LPA and Sph-1-P actions in platelet activation and found that LPA functioned as a much stronger agonist than Sph-1-P, and high concentrations of Sph-1-P specifically desensitized LPA-induced intracellular Ca²⁺ mobilization. In order to identify the responsible receptors underlying these observations, we analyzed the LPA and Sph-1-P receptors which might be expressed in human platelets, by RT-PCR. We found for the first time that Edg2, 4, 6 and 7 mRNA are expressed in human platelets.

【 授权许可】

Unknown