Platelet aggregating activity of lysophosphatidic acids is not related to their calcium ionophore properties

FEBS Letters

Chap, Hugues¹  Simon, Marie-Françoise¹  Douste-Blazy, Louis¹ 

[1] INSERM Unité 101, Biochimie des Lipides, Hôpital Purpan, 31059 Toulouse, France

关键词: Platelet;    Aggregation;    Activation;    Calcium;    Lysophosphatidic acid;    Ether phospholipid;    PA;    phosphatidic acid;    LPA;    lysophosphatidic acid;    P-GPA;    1-palmitoyl-sn-glycero-3-phosphate;    H-GPA;    1-hexadecyl-sn-glycero-3-phosphate;    HME;    half-maximal effect;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;   

DOI  :  10.1016/0014-5793(84)80055-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The calcium ionophore properties of A23187 and of two lysophosphatidic acid (LPA) analogs (1-palmitoyl- and 1-hexadecyl-sn-glycero-3-phosphate or P-GPA and H-GPA, respectively) were compared using platelet membrane vesicles loaded with ⁴⁵Ca. Half maximal effect (HME) was obtained at 5 μM and 10 μM for H-GPA and P-GPA, respectively, against 0.7 μM for A23187, which released 2 times more Ca. The three compounds also induced platelet aggregation with a HME at 0.5 μM, 0.3 μM and 0.01 μM for A23187, P-GPA and H-GPA, respectively. The clear dissociation between the two effects appearing for both LPA raises some doubt about the general idea that (lyso) PA participate in cell activation through their calcium ionophore properties.

【 授权许可】

Unknown