Inhibition by forskolin of cytosolic calcium rise, shape change and aggregation in quin2‐loaded human platelets

FEBS Letters

Sage, S.O.²  Rink, T.J.¹ 

[1] Smith, Kline and French Research Limited, The Frythe, Welwyn, AL6 9AR, England;The Physiological Laboratory, Downing Street, Cambridge, CB2 3EG England

关键词: Platelet;    Calcium;    Fluorescent indicator;    Forskolin;    Shape-change;    Aggregation;    cAMP;    adenosine 3'5'-cyclic monophosphate;    IBMX;    isobutylmethylxanthine;    PAF;    platelet activating factor (1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine);    [Ca2+]i;    cytoplasmic free calcium concentration;    DMSO;    dimethyl sulphoxide;   

DOI  :  10.1016/0014-5793(85)80890-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The adenylate cyclase stimulator forskolin was used to study the inhibitory effects of elevated cAMP on the activation of washed human platelets loaded with the fluorescent Ca²⁺ indicator quinl. In the presence of 10 μM isobutylmethylxanthine forskolin inhibited rises in [Ca²⁺]_i evoked by thrombin and platelet-activating factor (PAF) due to both Ca²⁺ influx and release from internal stores with similar potency. Aggregation evoked by thrombin and PAF was suppressed whilst partial shape-change persisted, even in the absence of a measurable rise in [Ca²⁺]_i. Forskolin did not affect the rise in [Ca²⁺ _i evoked by Ca²⁺ ionophore; aggregation was suppressed but shape-change persisted.

【 授权许可】

Unknown