Cloning of a novel putative G‐protein‐coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue

FEBS Letters

Schäfer, Martin²  Wang, Xioamin¹  Höllt, Volker¹  Vanetti, Mirko¹  Kouba, Monika¹ 

[1] Department of Physiology, University of Munich, Germany;Department of Anatomy, University of Mainz, Germany

关键词: G-protein-coupled receptor;    NLR;    NG108-15 cell;    Polymerase chain reaction;    BLR1;    Burkitt's lymphoma;    Interleukin 8;   

DOI  :  10.1016/0014-5793(93)80102-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel G-protein-coupled receptor was isolated from mouse and rat neuronal and lymphatic tissues. The amino acid sequence of the rat receptor (rNLR) shows an overall homology of 80% to a recently cloned receptor from Burkitt's lymphoma cells (BLR1) which is exclusively expressed in lymphatic tissues [(1992) Eur. J. Immunol. 22, 2795]. Much less homology between rNLR and BLR1 was observed at the N-terminus (about 40%), whereas rNLR and the mouse homologue mNLR show 92% amino acid identity. Northern blot analysis of NLR revealed a predominant 5.5 kb mRNA species in various brain regions and neuronal cell lines, whereas in the spleen a 3 kb transcript is predominant. This distribution suggests a role of NLR in the nervous and immune systems.

【 授权许可】

Unknown