Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis‐elements unrelated to canonical translation initiation signals

FEBS Letters

Sansone, Federica²  Antico, Giovanni²  Calogero, Raffaele A.²  Puca, Antimina²  Brandi, Anna¹  Andrè, Alessandra² 

[1] Dipartimento di Scienze Cliniche e Biologiche, Università di Torino, Ospedale S. Luigi, Regione Gonzole 10, 10043 Orbassano, Italy;Dipartimento di Genetica, Biologia Generale e Molecolare, Università Federico II di Napoli, via Mezzocannone 8, 80134 Naples, Italy

关键词: Combinatorial library;    Reinitiation;    Protein synthesis;    Eubacterium;   

DOI  :  10.1016/S0014-5793(00)01198-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Eubacteria, de novo translation of some internal cistrons may be inefficient or impossible unless the 5′ neighboring cistron is also translated (translational coupling). Translation reinitiation is an extreme case of translational coupling in which translation of a message depends entirely on the presence of a nearby terminating ribosome. In this work, the characteristics of mRNA cis-elements inducing the reinitiation process in Escherichia coli have been investigated using a combinatorial approach. A number of novel translational reinitiation sequences (TRSs) were thus identified, which show a wide range of reinitiation activities fully dependent on a translational coupling event and unrelated to the presence/absence of secondary structure or mRNA stability. Moreover, some of the isolated TRSs are similar to intercistronic sequences present in the E. coli genome.

【 授权许可】

Unknown