Influence of Sire Breed on the Interplay among Rumen Microbial Populations Inhabiting the Rumen Liquid of the Progeny in Beef Cattle

PLoS One

Mi Zhou¹  Laksiri A. Goonewardene¹  Emma Hernandez-Sanabria¹  Zhiquan Wang¹  Stephen S. Moore¹  Le Luo Guan² 

[1] Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada;The University of Queensland Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation, St. Lucia, Queensland, Australia

关键词: Diet;    Methanogens;    Bacteria;    Prevotella;    Cloning;    Eubacterium;    Beef;    Cattle;   

DOI  :  10.1371/journal.pone.0058461

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

This study aimed to evaluate whether the host genetic background impact the ruminal microbial communities of the progeny of sires from three different breeds under different diets. Eighty five bacterial and twenty eight methanogen phylotypes from 49 individuals of diverging sire breed (Angus, ANG; Charolais, CHA; and Hybrid, HYB), fed high energy density (HE) and low energy density (LE) diets were determined and correlated with breed, rumen fermentation and phenotypic variables, using multivariate statistical approaches. When bacterial phylotypes were compared between diets, ANG offspring showed the lowest number of diet-associated phylotypes, whereas CHA and HYB progenies had seventeen and twenty-three diet-associated phylotypes, respectively. For the methanogen phylotypes, there were no sire breed-associated phylotypes; however, seven phylotypes were significantly different among breeds on either diet (P<0.05). Sire breed did not influence the metabolic variables measured when high energy diet was fed. A correlation matrix of all pairwise comparisons among frequencies of bacterial and methanogen phylotypes uncovered their relationships with sire breed. A cluster containing methanogen phylotypes M16 (Methanobrevibacter gottschalkii) and M20 (Methanobrevibacter smithii), and bacterial phylotype B62 (Robinsoniella sp.) in Angus offspring fed low energy diet reflected the metabolic interactions among microbial consortia. The clustering of the phylotype frequencies from the three breeds indicated that phylotypes detected in CHA and HYB progenies are more similar among them, compared to ANG animals. Our results revealed that the frequency of particular microbial phylotypes in the progeny of cattle may be influenced by the sire breed when different diets are fed and ultimately further impact host metabolic functions, such as feed efficiency.

【 授权许可】

CC BY