Peroxide accumulation without major mitochondrial alteration in replicative senescence

FEBS Letters

Pfeuty, A.¹  Gueride, M.¹ 

[1]Université de Versailles/St Quentin-en-Yvelines, UPRESA-CNRS 8087, Bâtiment Fermat, 45 avenue des Etats-Unis, 78035 Versailles Cedex, France

关键词: Ageing;    Oxidative stress;    Mitochondrial membrane potential;    Chondrocyte;    ΔΨ;    transmembrane electric potential difference;    PBS;    phosphate buffered saline;    ROS;    reactive oxygen species;    DiOC6(3);    3;    3′-dihexyloxacarbocyanine;    NAO;    nonyl-acridine orange;    DCFH-DA;    2′;    7′-dichlorofluorescein diacetate;    FDA;    fluorescein diacetate;   

DOI  :  10.1016/S0014-5793(00)01188-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aim of this work was to determine how ageing changes were related to each other by studying the establishment of a senescent state in cell culture, rabbit articular chondrocytes. A striking increase of the amount of peroxides appeared at 2/3 of the time of cell growth, which is responsible for an oxidative stress, as shown by the appearance of oxidized proteins, the overexpression of HSP27 gene and the accumulation of HSP27 protein. While no change of the mitochondrial membrane potential was observed all along the cell culture, p21, a protein overproduced in senescent cells, appeared with the peak of peroxides and accumulated.

【 授权许可】

Unknown