Characterization of a novel endopolygalacturonase from Aspergillus niger with unique kinetic properties

FEBS Letters

Kester, Harry C.M.¹  Visser, Jaap¹  Pařenicová, Lucie¹  Benen, Jacques A.E.¹ 

[1] Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, Dreyenlaan 2, 6703 HA Wageningen, The Netherlands

关键词: Endopolygalacturonase;    Processivity;    Methylated oligogalacturonate;    Aspergillus niger;    PG;    endopolygalacturonase;    (GalpA)n;    oligogalacturonate with degree of polymerization (n);    r(GalpA)n;    reduced oligogalacturonate with degree of polymerization (n);    DE;    degree of esterification;    BCF;    bond cleavage frequency;   

DOI  :  10.1016/S0014-5793(00)01173-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated and characterized a new type of endopolygalacturonase (PG)-encoding gene, pgaD, from Aspergillus niger. The primary structure of PGD differs from that of other A. niger PGs by a 136 amino acid residues long N-terminal extension. Biochemical analysis demonstrated extreme processive behavior of the enzyme on oligomers longer than five galacturonate units. Furthermore, PGD is the only A. niger PG capable of hydrolyzing di-galacturonate. It is tentatively concluded that the enzyme is composed of four subsites. The physiological role of PGD is discussed.

【 授权许可】

Unknown