Characterization of Arabidopsis AtAMT2, a novel ammonium transporter in plants

FEBS Letters

Sohlenkamp, Christian¹  Shelden, Megan²  Udvardi, Michael¹  Howitt, Susan² 

[1] Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, Germany;Division of Biochemistry and Molecular Biology, The Australian National University, Canberra, ACT 0200, Australia

关键词: Ammonium transporter;    Methylammonium;    Nitrogen regulation;    Arabidopsis thaliana;    HATS;    high-affinity ammonium transporter;    LATS;    low-affinity ammonium transporter;    MA;    methylammonium;    YNB-N;    yeast nitrogen base without amino acids and ammonium sulfate;   

DOI  :  10.1016/S0014-5793(00)01153-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and characterized the first member of a novel family of ammonium transporters in plants: AtAMT2 from Arabidopsis thaliana. AtAMT2 is more closely related to bacterial ammonium transporters than to plant transporters of the AMT1 family. The protein was expressed and functionally characterized in yeast. AtAMT2 transported ammonium in an energy-dependent manner. In contrast to transporters of the AMT1 family, however, AtAMT2 did not transport the ammonium analogue, methylammonium. AtAMT2 was expressed more highly in shoots than roots and was subject to nitrogen regulation.

【 授权许可】

Unknown