Ammonium (methylammonium) is the co‐repressor of nitrate reductase in Chlamydomonas reinhardii

FEBS Letters

Fernández, Emilio¹  Franco, Antonio R.¹  Cárdenas, Jacobo¹ 

[1] Departamento de Bioquímica, Facultadde Ciencias, Universidad de Córdoba, 14005 Córdoba, Spain

关键词: Nitrate reductase;    Ammonium-repression;    Methylammonium;    Chlamydomonas reinhardii;    MSX;    L-methionine-DL-sulfoximine;   

DOI  :  10.1016/0014-5793(84)81217-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methylammonium cannot be used as a nitrogen source by the green alga Chlamydomonas reinhardii and, like ammonia, caused the repression of nitrate reductase without affecting the photosynthetic activity. Glutamine synthetase catalyzed the conversion ofmethylammonium to a single product, identified as γ-N-methylglutamine, which accumulated in the cells. Derepression of nitrate reductase was accompanied by a decrease in the intracellular concentration of methylammonium and a concomitant accumulation of γ-N-methylglutamine inside the cells. These facts strongly suggest that ammonium (methylammonium) per se, and not a product of its metabolism, is the co-repressor of nitrate reductase in C. reinhardii

【 授权许可】

Unknown