Drosophila MTN: a metazoan copper‐thionein related to fungal forms

FEBS Letters

Atrian, S.²  Bofill, R.³  Carrascal, M.¹  Romero-Isart, N.³  Capdevila, M.³  Abián, J.¹  Valls, M.²  Gonzàlez-Duarte, R.²  Gonzàlez-Duarte, P.³ 

[1] Departament de Bioanàlisis Mèdiques, Institut d'Investigacions Biomèdiques de Barcelona, CSIC, Jordi Girona Salgado 18, E-08034 Barcelona, Spain;Departament de Genètica, Facultat de Biologia, Av Diagonal 645, Universitat de Barcelona, E-08028 Barcelona, Spain;Departament de Quı́mica Inorgànica, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain

关键词: Cu(I) binding;    Cd(II) binding;    Zn(II) binding;    Molecular evolution;    Recombinant synthesis;    Drosophila metallothionein;    CD;    circular dichroism;    DTNB;    5;    5′-dithiobis(nitrobenzoic acid);    ICP-AES;    inductively coupled plasma atomic emission spectrometry;    ESI-MS;    electrospray ionization mass spectrometry;    MT;    metallothionein;   

DOI  :  10.1016/S0014-5793(00)01149-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two Drosophila metallothioneins (MT) have been reported: MTN, a 40 residue peptide including 10 Cys, and MTO, a 43 residue peptide including 12 Cys. However, neither functional nor evolutionary analyses for either of the Drosophila MT are available. Here, heterologous expression of Mtn in Escherichia coli is reported. The metal binding abilities of the Cu- and Zn-MTN complexes conformed in vivo, as well as the features of the Cd- and Cu-aggregates produced by metal replacement in vitro, have been determined by atomic emission spectrometry, circular dichroism and electrospray ionization mass spectrometry. Primary structure relationships with other MT have been examined. The results indicate a close resemblance of MTN to fungal copper-thioneins.

【 授权许可】

Unknown