MTO: the second member of a Drosophila dual copper‐thionein system

FEBS Letters

Atrian, S.²  González-Duarte, P.¹  Domenech, J.²  Capdevila, M.¹  Palacios, O.¹  Villarreal, L.¹ 

[1] Departament de Quı́mica, Facultat de Ciències, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain;Departament de Genètica, Facultat de Biologia, Av. Diagonal 645, Universitat de Barcelona, E-08028 Barcelona, Spain

关键词: Drosophila metallothionein;    Cu(I) binding;    Cd(II) binding;    Zn(II) binding;    Molecular evolution;    Recombinant synthesis;    CD;    circular dichroism;    ICP-AES;    inductively coupled plasma atomic emission spectroscopy;    ESI-MS;    electrospray ionization mass spectrometry;    MT;    metallothionein;    UV-vis;    electronic absorption;   

DOI  :  10.1016/S0014-5793(02)03754-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates.

【 授权许可】

Unknown