Alternative splicing within the TGF‐β type I receptor gene (ALK‐5) generates two major functional isoforms in vascular smooth muscle cells

FEBS Letters

Agrotis, Alex¹  Bobik, Alex¹  Condron, Melanie¹ 

[1] Cell Biology Laboratory, Baker Medical Research Institute, Alfred Hospital, Prahran, Vic. 8008, Australia

关键词: Type I transforming growth factor-β receptor (ALK-5);    Splice variant;    Vascular smooth muscle;   

DOI  :  10.1016/S0014-5793(00)01132-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-β type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length proteins differ from each other only by the in-frame presence or absence of Gly-Pro-Phe-Ser residues adjacent to their transmembrane domain. Stable expression of these alternate ALK-5 isoforms in ALK-5-deficient cells demonstrated that both were competent in signaling TGF-β-induced growth inhibition and gene transcription, but with an apparently distinct potency. Our data suggest that alternate splicing within the ALK-5 gene is an important mechanism whereby SMCs may regulate their response to TGF-β.

【 授权许可】

Unknown