Alternative splicing generates multiple mRNA forms of the acetylcholine receptor γ‐subunit in rat muscle

FEBS Letters

Villarroel, Alfredo¹ 

[1] Department of Physiology and Biophysics, Dalhousie University Faculty of Medicine, 3R1 Tupper Medical Building, Halifax, N.S. B3H 4H7, Canada

关键词: Endplate channel;    Muscle development;    Splice variant;   

DOI  :  10.1016/S0014-5793(99)00012-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fetal type acetylcholine receptor, composed of the αβγδ subunits, has shown a highly variable channel kinetics during postnatal development. We examine the hypothesis whether such a variability could result from multiple channel forms, differing in the N-terminus of the γ-subunit. RT-PCR revealed, in addition to the full-length mRNA, three new forms lacking exon 4. One of them in addition lacks 19 nucleotides from exon 5, predicting a complete subunit, with a 43 residues shorter N-terminus. A third one lacking the complete exon 5 predicts a subunit without transmembrane segments. These forms, generated by alternative splicing, may account for the kinetic variability of the acetylcholine receptor channel.

【 授权许可】

Unknown