Dominant negative myostatin produces hypertrophy without hyperplasia in muscle

FEBS Letters

Tidball, James G.²  Zhu, Xiaolei¹  Hadhazy, Michele¹  Wehling, Michelle²  McNally, Elizabeth M.¹ 

[1] Department of Medicine, Section of Cardiology, and Department of Human Genetics, University of Chicago, 5841 S. Maryland, MC 6088, Chicago, IL 60637, USA;Department of Physiological Science, University of California, Los Angeles, CA 90095, USA

关键词: Muscle development;    Muscle regeneration;    Muscular dystrophy;    TGF-β;   

DOI  :  10.1016/S0014-5793(00)01570-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myostatin, a TGF-β family member, is a negative regulator of muscle growth. Here, we generated transgenic mice that expressed myostatin mutated at its cleavage site under the control of a muscle specific promoter creating a dominant negative myostatin. These mice exhibited a significant (20–35%) increase in muscle mass that resulted from myofiber hypertrophy and not from myofiber hyperplasia. We also evaluated the role of myostatin in muscle degenerative states, such as muscular dystrophy, and found significant downregulation of myostatin. Thus, further inhibition of myostatin may permit increased muscle growth in muscle degenerative disorders.

【 授权许可】

Unknown