Chick embryo spinal cord neurons synthesize a transferrin‐like myotrophic protein

FEBS Letters

Stamatos, Cathryn¹  Fine, Richard E.¹  Squicciarini, Joseph¹ 

[1] Departments of Biochemistry and Physiology, Boston University School of Medicine, 80 East Concord Street, Boston, MA 02118, USA

关键词: Muscle development;    Acetylcholine receptor;    Cell culture;    Sciatin;    Transferrin;   

DOI  :  10.1016/0014-5793(83)80649-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Highly enriched cultures of chick embryo spinal cord neurons synthesize and secrete a protein which is immunoprecipitable by anti-ovotransferrin. Ovotransferrin, an iron-binding glycoprotein of M _r 80 000, is also shown to stimulate in vitro myogenesis of cultured chick embryo myotubes as measured by saturable dose-dependent increase in acetylcholine receptors. This effect is probably dependent on ovotransferrin's ability to donate iron to the cells. In many respects ovotransferrin is similar to ‘sciatin’, a myotrophic protein isolated from chicken sciatic nerves [Proc. Natl. Acad. Sci. USA (1980) 77, 6922–6925].

【 授权许可】

Unknown