The open reading frame of the Na+‐dependent glutamate transporter GLAST‐1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain

FEBS Letters

Vaughan-Thomas, Anne¹  Huggett, Jim¹  Mason, Deborah¹ 

[1] School of Bioscience, Cardiff University, Museum Avenue, P.O. Box 911, Cardiff CF10 3US, UK

关键词: GLAST-1;    Bone;    Glutamate;    Transporter;    Splice variant;   

DOI  :  10.1016/S0014-5793(00)02175-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously we have reported expression of an mRNA with homology to the Na⁺-dependent glutamate transporter, GLAST-1, in bone. Here we demonstrate that the complete open reading frame of GLAST-1 mRNA and corresponding 69 kDa protein are expressed in rat bone in vivo. We have also discovered a novel splice variant (GLAST-1a), lacking exon 3, expressed in rat bone and brain. A 55 kDa protein detected by anti-GLAST antibody in rat cerebellum corresponds to the molecular weight of unglycosylated GLAST-1a. This has led us to propose that GLAST-1a has an opposite orientation in the cell membrane to GLAST-1.

【 授权许可】

Unknown