Identification of a melanoma antigen, PRAME, as a BCR/ABL‐inducible gene

FEBS Letters

Takeshita, Akihiro²  Nagamura-Inoue, Tokiko³  Fukushima, Toshihiro¹  Asano, Shigetaka³  Nagamura, Fumitaka³  Tojo, Arinobu³  Watari, Kiyoshi³  Tani, Kenzaburo³  Motoji, Toshiko⁴ 

[1] The First Department of Internal Medicine, Fukui Medical School, 23-3 Shimogoozuki, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan;The Third Department of Internal Medicine, Hamamatsu Medical School, 3600 han-da-cho, Hamamatsu 431-3192, Japan;Department of Hematology-Oncology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan;Department of Hematology-Oncology, Tokyo Women's Medical School, 8-1 kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan

关键词: Differential display;    PRAME;    BCR/ABL;    Chronic myeloid leukemia;    Blastic crisis;    Philadelphia+-acute lymphoblastic leukemia;   

DOI  :  10.1016/S0014-5793(00)01112-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to elucidate molecular events in BCR/ABL-induced transformation, we adopted a polymerase chain reaction (PCR)-based technique of differential display and compared mRNA expression in human factor-dependent cells, TF-1, with that in factor-independent cells, ID-1, which were established from TF-1 cells by transfection of BCR/ABL. Cloning and sequencing of a gene which was upregulated in ID-1 cells revealed that the gene was identical to a melanoma antigen, PRAME. Our present study demonstrated that PRAME was markedly expressed in primary leukemic cells with chronic myeloid leukemia (CML) in blastic crisis and Philadelphia (Ph)⁺-acute lymphoblastic leukemia (ALL), in which BCR/ABL played an important role as a pathogenic gene. Moreover, comparison of PRAME expression among CD34⁺ cells with CML in blastic, accelerated, and chronic phases revealed a higher expression in CML in advanced phases. Thus PRAME was considered to be a good candidate for a marker of Ph⁺-leukemic blast cells as well as a new target antigen of leukemic blast cells that cytotoxic T cells can recognize.

【 授权许可】

Unknown