Functional association between the nef gene product and gag‐pol region of HIV‐1

FEBS Letters

Ishimoto, Akinori¹  Ono, Takeshi¹  Iwatani, Yasumasa¹  Nishimura, Akiko¹  Sakai, Hiroyuki¹ 

[1] Laboratory of Gene Analysis, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan

关键词: Human immunodeficiency virus type 1;    Nef;    Gag;    Pol;    HIV-1;    human immunodeficiency virus type 1;    MAGI;    multinuclear activation of a galactosidase indicator;    RT;    reverse transcriptase;    WT;    wild type;    GFP;    green fluorescent protein;   

DOI  :  10.1016/S0014-5793(99)01791-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nef gene function is diverse among virus isolates of primate immunodeficiency viruses. We found differential effects of nef mutation on the virus replication between two HIV-1 clones, NL432 and LAI. The nef mutation in NL432 affected the infectivity more severely compared with that in LAI, although the Nef functions of both clones were comparable. Analysis with a series of chimeric viruses between NL432 and LAI revealed that the gag-pol region was responsible for the differential effect of nef mutation. The functional association between Nef and gag-pol suggested that one of the potential targets of Nef was located within the gag-pol region.

【 授权许可】

Unknown