FEBS Letters | |
Functional association between the nef gene product and gag‐pol region of HIV‐1 | |
Ishimoto, Akinori1  Ono, Takeshi1  Iwatani, Yasumasa1  Nishimura, Akiko1  Sakai, Hiroyuki1  | |
[1] Laboratory of Gene Analysis, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan | |
关键词: Human immunodeficiency virus type 1; Nef; Gag; Pol; HIV-1; human immunodeficiency virus type 1; MAGI; multinuclear activation of a galactosidase indicator; RT; reverse transcriptase; WT; wild type; GFP; green fluorescent protein; | |
DOI : 10.1016/S0014-5793(99)01791-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Nef gene function is diverse among virus isolates of primate immunodeficiency viruses. We found differential effects of nef mutation on the virus replication between two HIV-1 clones, NL432 and LAI. The nef mutation in NL432 affected the infectivity more severely compared with that in LAI, although the Nef functions of both clones were comparable. Analysis with a series of chimeric viruses between NL432 and LAI revealed that the gag-pol region was responsible for the differential effect of nef mutation. The functional association between Nef and gag-pol suggested that one of the potential targets of Nef was located within the gag-pol region.
【 授权许可】
Unknown
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