Clustered localization of oligomeric Nef protein of human immunodeficiency virus type 1 on the cell surface

FEBS Letters

Yamamoto, Naoki¹  Adachi, Akio³  Fujita, Yoshikazu²  Otake, Kaori⁴  Nagai, Yoshiyuki⁵  Fujii, Yoichi⁴  Tashiro, Masato⁶ 

[1] Department of Microbiology, School of Medicine, Tokyo Medical and Dental University, Tokyo 113, Japan;Equipment Center for Research and Education, Nagoya University School of Medicine, Nagoya 466, Japan;Department of Virology, Tokushima University School of Medicine, Tokushima 770, Japan;Institute for Laboratory Animal Research, Nagoya University School of Medicine, Nagoya 466, Japan;Department of Viral Infection, Institute of Medical Science, University of Tokyo, Tokyo 108, Japan;Department of Virology 1, National Institute of Health, Tokyo 162, Japan

关键词: Human immunodeficiency virus type 1;    Nef;    Env;   

DOI  :  10.1016/0014-5793(96)01048-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied human immunodeficiency virus type 1 (HIV-1) Nef protein biochemically and histologically. HIV-1 Nef, derived from baculosystem and from cells infected with HIV-1, formed homomeric monomers, dimers, trimers, and further polymers. These oligomers were non-covalently associated. In cells infected with HIV-1, Nef molecules were clustered at the cell surface as well as cytoplasm. Our previous results have indicated that the Nef on the surface of cells infected with HIV-1 is cytotoxic against uninfected CD4+ T cells. Thus, it is very likely that the HIV-1-mediated cytotoxic reaction is due, at least in part, to the clustered localization of oligomeric Nef on the cell surface.

【 授权许可】

Unknown