HIV‐1 capsid mutants inhibit the replication of wild‐type virus at both early and late infection phases

FEBS Letters

Adachi, Akio³  Shimano, Reika³  Hatanaka, Masakazu²  Kawamura, Meiko¹  Ogasawara, Takashi³  Akari, Hirofumi³  Furuta, Rika A¹  Inubushi, Ritsuko³  Amano, Kazushi³ 

[1] Institute for Virus Research, Kyoto University, Kyoto 606, Japan;Shionogi Institute for Medical Science, Osaka 566, Japan;School of Medicine, University of Tokushima, Tokushima 770, Japan

关键词: Human immunodeficiency virus type 1;    Gag;    Capsid;    Matrix;    Nucleocapsid;   

DOI  :  10.1016/S0014-5793(97)01132-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In-frame mutations were introduced into various portions of the human immunodeficiency virus type 1 (HIV-1) gag gene, and potentials of the mutants to suppress the replication of wild-type HIV-1 were monitored. In contrast to results obtained with matrix and nucleocapsid mutants, almost all capsid mutants blocked HIV-1 replication completely in single-round replication assays. A capsid mutant designated C6b was demonstrated to be one of the most efficient inhibitors for HIV-1 reported to date, and to be effective at both early and late viral replication phases. T-cells, which are engineered to express the C6b Gag in response to HIV-1 infection, were perfectly resistant to HIV-1.

【 授权许可】

Unknown