Cloning, expression and characterization of a novel four EF‐hand Ca2+‐binding protein from olive pollen with allergenic activity

FEBS Letters

Rodrı́guez, Rosalı́a¹  Villalba, Mayte¹  Ledesma, Amalia¹ 

[1] Departamento de Bioquı́mica y Biologı́a Molecular, Facultad de Quı́mica, Universidad Complutense, 28040 Madrid, Spain

关键词: Ca2+-binding protein;    Olive pollen allergen;    Ole e 8;    Amino acid sequence;    Recombinant production;    CaBP;    Ca2+-binding protein;    CaM;    calmodulin;    CD;    circular dichroism;   

DOI  :  10.1016/S0014-5793(99)01790-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel allergenic member of the family of Ca²⁺-binding proteins has been cloned from olive tree pollen. The isolated DNA codes for a protein of 171 amino acid residues, which displays four EF-hand sequence motifs. The encoded protein was overproduced in Escherichia coli and purified. The protein (18 795 Da), which binds Ca²⁺ and IgE antibodies from patients allergic to olive pollen, undergoes Ca²⁺-dependent conformational changes. It is retained on a phenyl-Sepharose column, which indicates the existence of regulatory EF-hand domains. This fact suggests its involvement in Ca²⁺-dependent signal transduction events of the pollen grain. This allergen could be considered as a member of a new subfamily of EF-hand Ca²⁺-binding proteins since it displays a low amino acid sequence similarity with the so far known proteins.

【 授权许可】

Unknown