Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC

FEBS Letters

Mann, Karlheinz¹  Deutzmann, Rainer¹  Paulsson, Mats¹  Timpl, Rupert¹ 

[1] Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG

关键词: Extracellular matrix;    Ca2+-binding protein;    Amino acid sequence;    Proteolytic fragment;   

DOI  :  10.1016/0014-5793(87)81040-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Up to 80% of the calcium-binding protein BM-40 could be extracted from a tumor basement membrane with a physiological buffer containing 10 mM EDTA. About half of its amino acid sequence was determined by Edman degradation demonstrating identity with cDNA deduced sequences of bone osteonectin and SPARC.

【 授权许可】

Unknown