Complete amino acid sequence of the sarcoplasmic calcium‐binding protein (SCP‐I) from crayfish (Astacus leptodactilus)

FEBS Letters

Cox, J.A.²  Jauregui-Adell, J.¹  Wnuk, W.² 

[1] Centre de Recherches de Biochimie Macromoléculaire du Centre National de la Recherche Scientifique, Boîte Postale 5051, F-34033 Montpellier Cedex, France;Département de Biochimie, Université de Genève, Quai Ernest-Ansermet 30, CH-1211 Genève 4, Switzerland

关键词: Chain;    α-;    Sarcoplasmic protein;    Ca2+-binding protein;    Amino acid sequence;    (Astacus leptodactylus);    PV;    parvalbumin;    SCP;    sarcoplasmic calcium-binding protein;    CaBP;    calcium-binding protein;   

DOI  :  10.1016/0014-5793(89)80131-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequence of the alpha chain of the dimeric sarcoplasmic Ca²⁺-binding protein (SCP-I=α₂) from crayfish (Astacus leptodactylus) has been determined by partial automatic sequencing of the peptides derived from tryptic digests of the protein after citraconylation or treatment with 1,2-cyclohexanedione. Overlapping peptides were obtained by cleavage with o-iodosobenzoic acid, or digestion with Staphylococcus aureus protease, thermolysin and pepsin. The acetylated N-terminus was identified by fast atom bombardment mass spectrometry. The monomeric protein contains 192 amino acids and has an M _r of 21 643. The sequence shows the presence of three calcium-binding sites and perhaps of two others that may be degenerated.

【 授权许可】

Unknown