Zintrons in rat α‐lactalbumin gene

FEBS Letters

Brahmachari, Samir K.¹  Meera, G.¹  Ramesh, N.¹ 

[1] Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India

关键词: Lactalbumin;    α-;    Intron sequence;    DNA;    Z-;    DNA supercoiling;    (Rat);   

DOI  :  10.1016/0014-5793(89)81463-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The eukaryotic genome is characterised by the occurrence of a large amount of repetitive DNA which exists within and around coding sequences. Random repeats of (TG) _n sequences have been observed in several introns. In this paper we show that (TG)₁₄ and (TG)₂₄ sequences present in the third intron of rat α-lactalbumin gene adopt left-handed Z-helices under varying superhelical densities. The overall sequence of the parent plasmid further influenced the level of supercoiling at which the B to Z transition could be induced in (TG) _n sequences. Such Z-potential intervening sequences (zintrons) could act as buffers to maintain desired levels of supercoiling near the transcribed sequences.

【 授权许可】

Unknown