Ozone potentiates vitamin E depletion by ultraviolet radiation in the murine stratum corneum

FEBS Letters

Packer, Lester²  Cross, Carroll E.³  Luu, Chate²  Valacchi, Giuseppe¹  Weber, Stefan U.² 

[1] Institute of General Physiology, University of Siena, Siena, Italy;Department of Molecular and Cell Biology, University of California, 251 Life Sciences Addition, Berkeley, CA 94720-3200, USA;Departments of Medicine and Physiology, University of California, Davis, CA, USA

关键词: Reactive oxygen species;    Antioxidant;    Malondialdehyde;    Peroxidation;    SSUV;    solar simulated ultraviolet;    ROS;    reactive oxygen species;    MDA;    malondialdehyde;    SC;    stratum corneum;    MED;    minimal erythemal dose;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/S0014-5793(99)01787-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As the outermost layer of the skin, the stratum corneum is exposed to environmental oxidants. To investigate putative synergisms of environmental oxidative stressors in stratum corneum, hairless mice were exposed to ultraviolet radiation (UV) and ozone (O₃) alone and in combination. Whereas a significant depletion of α-tocopherol was observed after individual exposure to either a 0.5 minimal erythemal dose of UV or 1 ppm O₃ for 2 h, the combination did not increase the effect of UV alone. However, a dose of 0.5 ppm O₃×2 h, which had no effect when used alone, significantly enhanced the UV-induced depletion of vitamin E. We conclude that concomitant exposure to low doses of UV and O₃ at levels near those that humans can be exposed to causes additive oxidative stress in the stratum corneum.

【 授权许可】

Unknown