Functional expression of His‐tagged sensory rhodopsin I in Escherichia coli

FEBS Letters

Chizhov, Igor¹  Schmies, Georg¹  Engelhard, Martin¹ 

[1] Max-Planck-Institut für Molekulare Physiologie, Otto-Hahn Straße 11, D-44227 Dortmund, Germany

关键词: Archaeal phototaxis;    Photocycle;    Halobacterium salinarum;    DM;    dodecylmaltoside;    IPTG;    isopropylthiogalactoside;    MES;    morpholinoethanesulfonic acid;    SRI;    sensory rhodopsin I;    SRII;    sensory rhodopsin II;   

DOI  :  10.1016/S0014-5793(99)01760-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sensory rhodopsin I (SRI) from Halobacterium salinarum was functionally expressed in Escherichia coli and subsequently purified to homogeneity using a C-terminal His-tag anchor. Yields of 3–4 mg SRI/l cell culture can be obtained. The absorption and photocycle properties of SRI were similar if not indistinguishable from those of the homologously expressed SRI. A global fit analysis of the photocycle data and the calculation of the spectra of states provided strong evidence for the existence of an N-like intermediate.

【 授权许可】

Unknown