Resonance Raman spectroscopy of sensory rhodopsin II from Natronobacterium pharaonis

FEBS Letters

Lüttenberg, Beate³  Hildebrandt, Peter²  Engelhard, Martin³  Gellini, Cristina¹  Sydor, Jens³ 

[1] Laboratorio di Spettroscopia Molecolare, Dipartimento di Chimica, Universita’ di Firenze, Via G. Capponi 9, 1-50121 Firenze, Italy;Max-Planck-Institut für Strahlenchemie, Stiftstraße 34-36, D-45410 Mülheim an der Ruhr, Germany;Max-Planck-Institut für Molekulare Physiologie, Otto-Hahn Straße 11, D-44227 Dortmund, Germany

关键词: Archaeal phototaxis;    Deuterium shift;    Photocycle;    Schiff base;    RR;    resonance Raman;    pSRII;    sensory rhodopsin II (from Natronobacterium pharaonis);   

DOI  :  10.1016/S0014-5793(00)01472-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sensory rhodopsin II (pSRII), the photophobic receptor from Natronobacterium pharaonis, has been studied by time-resolved resonance Raman (RR) spectroscopy using the rotating cell technique. Upon excitation with low laser power, the RR spectra largely reflect the parent state pSRII₅₀₀ whereas an increase of the laser power leads to a substantial accumulation of long-lived intermediates contributing to the RR spectra. All RR spectra could consistently be analysed in terms of four component spectra which were assigned to the parent state pSRII₅₀₀ and the long-lived intermediates M₄₀₀, N₄₈₅ and O₅₃₅ based on the correlation between the C=C stretching frequency and the absorption maximum. The parent state and the intermediates N₄₈₅ and O₅₃₅ exhibit a protonated Schiff base. The C=N stretching frequencies and the H/D isotopic shifts indicate strong hydrogen bonding interactions of the Schiff base in pSRII₅₀₀ and O₅₃₅ whereas these interactions are most likely very weak in N₄₈₅.

【 授权许可】

Unknown