The RosR transcription factor is required for gene expression dynamics in response to extreme oxidative stress in a hypersaline-adapted archaeon

BMC Genomics

Research Article

Nicholas Gillum¹  J Lomax Boyd¹  Kriti Sharma¹  Amy Schmid¹ 

[1] Center for Systems Biology, Institute for Genome Sciences and Policy, 27710, Durham, NC, USA;Biology Department, Duke University, 27710, Durham, NC, USA;

关键词: Halobacterium salinarum;    Oxidative stress;    Gene regulation;    Transcription factor;    Archaea;   

DOI  :  10.1186/1471-2164-13-351

 received in 2012-01-04, accepted in 2012-07-05,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundPrevious work has shown that the hypersaline-adapted archaeon, Halobacterium salinarum NRC-1, is highly resistant to oxidative stress caused by exposure to hydrogen peroxide, UV, and gamma radiation. Dynamic alteration of the gene regulatory network (GRN) has been implicated in such resistance. However, the molecular functions of transcription regulatory proteins involved in this response remain unknown.ResultsHere we have reanalyzed several existing GRN and systems biology datasets for H. salinarum to identify and characterize a novel winged helix-turn-helix transcription factor, VNG0258H, as a regulator required for reactive oxygen species resistance in this organism. This protein appears to be unique to the haloarchaea at the primary sequence level. High throughput quantitative growth assays in a deletion mutant strain implicate VNG0258H in extreme oxidative stress resistance. According to time course gene expression analyses, this transcription factor is required for the appropriate dynamic response of nearly 300 genes to reactive oxygen species damage from paraquat and hydrogen peroxide. These genes are predicted to function in repair of oxidative damage to proteins and DNA. In vivo DNA binding assays demonstrate that VNG0258H binds DNA to mediate gene regulation.ConclusionsTogether these results suggest that VNG0258H is a novel archaeal transcription factor that regulates gene expression to enable adaptation to the extremely oxidative, hypersaline niche of H. salinarum. We have therefore renamed VNG0258H as RosR, for r eactive o xygen s pecies r egulator.

【 授权许可】

Unknown   
© Sharma et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.