Identification of a cis‐acting element that interferes with thyroid hormone induction of the neurogranin (NRGN) gene

FEBS Letters

Martı́nez de Arrieta, Cruz¹  Bernal, Juan¹  Coloma, Antonio¹  Manzano, Jimena¹  Morte, Beatriz¹ 

[1] Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Arturo Duperier 4, 28029 Madrid, Spain

关键词: Thyroid hormone;    Brain gene expression;    Development;    Nuclear receptor;    Southwestern blotting;   

DOI  :  10.1016/S0014-5793(99)01706-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The neuron-specific RC3/neurogranin gene is regulated by thyroid hormone at the transcriptional level in brain and in cultured neuronal cells. Regulation in vivo displays exquisite regional selectivity which is not due to differential distribution of thyroid receptors and is most probably related to region-specific trans-acting elements. We have previously identified an intronic thyroid hormone responsive element in the human RC3 gene homolog, NRGN. In a search for cis-acting elements that might contribute to the specificity of thyroid regulation, we have identified a novel sequence, TTCCAAAATGG, which binds to a developmentally regulated protein, and interferes with T3 transactivation.

【 授权许可】

Unknown