Distinct energy requirement for nuclear import and export of importin β in living cells

FEBS Letters

Yoneda, Yoshihiro¹  Kose, Shingo¹  Imamoto, Naoko¹ 

[1] Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan

关键词: Importin;    Nuclear import;    Nuclear export;    Nuclear pore complex;    GFP;    green fluorescent protein;    HVJ;    hemagglutinating virus of Japan;    NES;    nuclear export signal;    NLS;    nuclear localization signal;    NPC;    nuclear pore complex;    RanGTP;    GTP-bound form of Ran;    TR;    Texas red;   

DOI  :  10.1016/S0014-5793(99)01641-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Importin β can shuttle between the nucleus and cytoplasm through the nuclear pore complex (NPC). This study deals with the issue of how the energy is utilized during the NPC passage of importin β. In chilled or ATP-depleted cells, importin β was transported into the nucleus, while the nuclear export of importin β was inhibited. Further, it was found that the nuclear export inhibition of importin β is not due to nuclear retention via binding to nucleoporins or nuclear importin α. These data show that the nuclear export of importin β involves energy-requiring step(s) in living cells.

【 授权许可】

Unknown