A novel glycosyltransferase with a polyglutamine repeat; a new candidate for GD1α synthase (ST6GalNAc V)

FEBS Letters

Nakanishi, Hayao¹  Shimizu, Nobuyuki¹  Kono, Mari²  Narimatsu, Hisashi³  Ikehara, Yuzuru¹  Kitamura, Tabito¹  Nishihara, Shoko³  Tatematsu, Masae¹  Tsuji, Shuichi² 

[1] Division of Pathology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464-0021, Japan;The Department of Glyco Molecular Biology, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-01, Japan;Division of Cell Biology, Institute of Life Science, Soka University, Hachioji, Tokyo 192-8857, Japan

关键词: GalNAcα2;    6-sialyltransferase;    ST6GalNAc V;    GD1α synthase;    Polyglutamine repeat;    Central nervous system;    NeuAc;    N-acetylneuraminic acid;    GalNAc;    N-acetylgalactosamine;    ST6GalNAcs;    GalNAcα2;    6-sialyltransferases;    ST6GalNAc I;    GalNAcα2;    6-sialyltransferase I (EC 2.4.99.3);    ST6GalNAc II;    Galβ1;    3GalNAc specific GalNAcα2;    6-sialyltransferase;    ST6GalNAc III;    the first type of NeuAcα2;    3-Galβ1;    3GalNAc specific GalNAc-α2;    6-sialyltransferase III (EC 2.4.99.7);    ST6GalNAc IV;    the second type of NeuAcα2;    3-Galβ1;    3GalNAc specific GalNAc-α2;    6-sialyltransferase IV (EC 2.4.99.7);    ST3Gal I;    Galβ1;    3GalNAcα2;    3-sialyltransferase (EC 2.4.99.4);    CMP-NeuAc;    cytidine 5′-monophospho-N-acetylneuraminic acid;    ORF;    open reading frame;    EST;    expressed sequence tags;    PCR;    polymerase chain reaction;    SDS-PAGE;    sodium dodecylsulfate-polyacrylamide gel electrophoresis;    TLC;    thin layer chromatography;    HPTLC;    high performance thin layer chromatography;    mAb;    monoclonal antibody;    NANase;    N-acetylneuraminidase;    The nomenclature for gangliosides follows the system of Svennerholm [26];    The abbreviated nomenclature for cloned sialyltransferases follows the system of Tsuji et al. [27];   

DOI  :  10.1016/S0014-5793(99)01605-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fifth type GalNAcα2,6-sialyltransferase (mST6GalNAc V) was cloned from a mouse brain cDNA library. mST6GalNAc V exhibited type II transmembrane topology containing a polyglutamine repeat, which showed 42.6% and 44.8% identity to mouse ST6GalNAc III and IV, respectively. Northern blot analysis revealed that the mST6GalNAc V gene was specifically expressed in forebrain and cerebellum. mST6GalNAc V exhibited GD1α synthetic activity from GM1b the same as mST6GalNAc III and IV. The activity ratio of GM1b toward fetuin and the expression pattern were completely different among the three ST6GalNAcs. Interestingly, the polyglutamine repeat number was different from that of inbred mice. We report the first glycosyltransferase with a polymorphic polyglutamine repeat.

【 授权许可】

Unknown