Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

FEBS Letters

Macfarlane, Wendy M.¹  Elrick, Lucy J.¹  Shennan, Kathleen I.J.¹  Campbell, Susan C.¹  Cragg, Helen¹  Docherty, Kevin¹ 

[1] Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK

关键词: Insulin gene;    Diabetes mellitus;    Amylin;    Transcription;   

DOI  :  10.1016/S0014-5793(99)01584-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic β-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4. The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus. Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at −253 to −244, and the islet amyloid polypeptide promoter through a sequence element located downstream of −138. The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2–230 and 231–349.

【 授权许可】

Unknown