FEBS Letters | |
Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters | |
Macfarlane, Wendy M.1  Elrick, Lucy J.1  Shennan, Kathleen I.J.1  Campbell, Susan C.1  Cragg, Helen1  Docherty, Kevin1  | |
[1] Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK | |
关键词: Insulin gene; Diabetes mellitus; Amylin; Transcription; | |
DOI : 10.1016/S0014-5793(99)01584-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic β-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4. The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus. Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at −253 to −244, and the islet amyloid polypeptide promoter through a sequence element located downstream of −138. The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2–230 and 231–349.
【 授权许可】
Unknown
【 预 览 】
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RO201912020308715ZK.pdf | 229KB | download |