Changes in myosin heavy chain mRNA and protein isoforms in single fibers of unloaded rat soleus muscle

FEBS Letters

Stevens, Laurence¹  Pette, Dirk¹  Gohlsch, Bärbel¹  Mounier, Yvonne² 

[1] Faculty of Biology, University of Konstanz, D-78457 Konstanz, Germany;Laboratoire de Plasticité Neuromusculaire, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq, France

关键词: Hindlimb suspension;    mRNA;    Myosin heavy chain isoform;    Reverse transcriptase polymerase chain reaction;    Single fiber study;    Slow-to-fast transition;    DTT;    dithiothreitol;    MHC;    myosin heavy chain;    RT-PCR;    reverse transcriptase polymerase chain reaction;    SDS-PAGE;    sodium dodecylsulfate polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(99)01596-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Changes in myosin heavy chain (MHC) mRNA and protein isoforms were investigated in single fibers from rat soleus muscle unloaded by hindlimb suspension for 4 and 7 days. Dramatic changes were seen after 4 days, when all fibers co-expressed slow and fast MHC mRNAs. Most fibers contained mRNAs for MHCIβ, MHCIIa, MHCIId(x), and MHCIIb. The up-regulation of the fast isoforms was only partially transmitted to the protein level. Atypical combinations of MHC mRNA isoforms, which deviated from the ‘next-neighbor rule’, were frequent in fibers from unloaded soleus. These atypical combinations increased with time and were not observed in the controls. The results suggest that hindlimb suspension elicits in soleus muscle pronounced perturbations in the expression of MHC isoforms by disrupting transcriptional and translational activities.

【 授权许可】

Unknown